From what? from a dvd, you can make an ISO via CloneDVD2, DVDDecrypter,DVDShrink. From already ripped dvds (VIDEO_TS, AUDIO_TS) you can create ISOs with CloneDVD2, DVDShrink, ImgTool Classic
Again : from what media? DVD or CD? since yer postin in the DVD section, Im assuming from a DVD. Thus you can use DVDDecrypter to rip the game itself (using ISO mode) Alcohol 120% might work but I dont do DVD game rips so I dunno how to do Game ISOs with Alcohol 120%